上海恒遠生物長期提供酶免Elisa試劑盒、ELISA檢測試劑盒、免疫組化試劑盒、人Elisa試劑盒、大小鼠Elisa試劑盒、抗體抗原，價格實惠，質量有保證,信譽*。凡購買目錄本公司ELISA檢測試劑盒可免費提供代測服務。更多Elisa試劑盒，咨詢：,.

試劑名稱：人鳥苷酸交換因子（GEF）

英文名稱：Human Guanine Exchange Factor,GEF ELISA KIT

檢測范圍：詳見說明書

產品規(guī)格：96人份/48人份

檢測目標：人、大小鼠、豬、豚鼠、雞、羊、兔...

保存條件： 2-8℃

應用范圍：科研用檢測試劑
人鳥苷酸交換因子（GEF）Assay procedure（操作步驟）
1.Dilute and add sample:Dilute Original density Standard as follow table:
8 pg/ml 5 Standard 150μl Original density Standard+150μl Standard diluent
4pg/ml 4 Standard 150μl 5 Standard+150μl Standard diluent
2 pg/ml 3 Standard 150μl 4 Standard+150μl Standard diluent
1 pg/ml 2 Standard 150μl 3 Standard +150μl Standard diluent
0.5 pg/ml 1 Standard 150μl 2 Standard +150μl Standard diluent
2.add sample：Set blank wells separay （blank comparison wells don’t add sample and HRP-
Conjugate reagent, other each step operation is same）. testing sample well. add Sample
dilution 40μl to testing sample well, then add testing sample 10μl （sample final
dilution is 5-fold）, add sample to wells , don’t touch the well wall as far as
possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold wash solution diluted 30-fold （or 20-fold） with distilled
water and reserve.
5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer
to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except blank well.
7.incubate：Operation with 3.
8.washing：Operation with 5.
9.color：Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the
light preservation for 15 min at 37℃
10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction（the blue color
change to yellow color）.
11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution
and within 15min.
相關產品：
人鳥苷酸交換因子ELISA試劑盒 Human Guanine Exchange Factor,GEF ELISA KIT
人維生素D受體ELISA試劑盒 Human Vitamin D Receptor,VD R ELISA KIT
人毒性休克綜合征毒素1ELISA試劑盒 Human toxic shock syndrome toxin,TSST-1 ELISA KIT
人金葡菌腸毒素ELISA試劑盒 Human staphylococcus aureus enterotoxins,SE ELISA KIT
人鈣聯(lián)蛋白ELISA試劑盒 Human calnexin,CNX ELISA KIT
人繆勒管抑制物質/抗繆勒管激素ELISA試劑盒 Human Mullerian Inhibiting Substance/Anti-Mullerian hormone,MIS/AMH ELISA KIT
人β細胞素ELISA試劑盒 Human beta cellulin,BTC ELISA KIT
人胎球蛋白AELISA試劑盒 Human Fetuin A,FETU-A ELISA KIT
人胎盤核糖核酸抑止劑ELISA試劑盒 Human placental ribonuclease inhibitor,HPRI ELISA KIT
人胎盤蛋白ELISA試劑盒 Human placental protein,PP ELISA KIT
人胎盤催乳素ELISA試劑盒 Human placenta lactogen,HPL ELISA KIT
人胎兒血紅蛋白ELISA試劑盒 Human fetal hemoglobin,HBF ELISA Kit
人抗巨細胞病毒抗體IgMELISA試劑盒 Human anti-cytomegalovirus IgM antibody,anti-CMV IgM ELISA KIT
人抗弓形體IgM抗體ELISA試劑盒 Human anti-toxoplasma IgM antibody,anti-tox IgM ELISA Kit
人抗風疹病毒IgG抗體ELISA試劑盒 Human anti-rubella virus IgG antibody,anti-RV IgG ELISA KIT